Decoding the Antibody Repertoire

High Throughput Sequencing of Multiple Transcripts from Single B Cells

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Éditeur :

Springer


Collection :

Springer Theses

Paru le : 2017-05-29



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Description

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.
Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells.

The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Pages
87 pages
Collection
Springer Theses
Parution
2017-05-29
Marque
Springer
EAN papier
9783319585178
EAN PDF
9783319585185

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
8
Taille du fichier
3400 Ko
Prix
94,94 €
EAN EPUB
9783319585185

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
8
Taille du fichier
1966 Ko
Prix
94,94 €