Artificially Controllable Nanodevices Constructed by DNA Origami Technology

Photofunctionalization and Single-Molecule Analysis

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Éditeur :

Springer


Collection :

Springer Theses

Paru le : 2015-12-16



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Description

In this book, the author deals mainly with two topics: (1) single-molecule visualization of switching behaviors in the DNA nanoframe system utilizing different kinds of molecular switches through the use of high-speed atomic force microscope (AFM); (2) construction of photocontrollable DNA nanostructures in programmed patterns and direct visualization of the dynamic assembling process. Here, high-speed AFM was employed to observe the dynamic movements of single molecules. Compared to a traditional single-molecule analysis method, such as fluorescence spectroscopy or electron microscopy, high-speed AFM makes possible the real-time observation of molecule behaviors. DNA nanostructures were designed and assembled as scaffolds to incorporate interested biomolecules. The observations were carried out under robust conditions without complicated pretreatment. Moreover, the photoresponsive molecules were successfully assembled into around 100 nm-sized DNA nanostructures. The assembly/disassembly of nanostructures can be regulated reversibly by photoirradiation. This book explains how DNA origami has gradually become a useful tool for the investigation of biochemical interactions in defined nanospace. It also shows the possibility of DNA nanostructures acting as nanodevices for application in biological systems, serving as a good introduction to basic DNA nanotechnology.
Pages
76 pages
Collection
Springer Theses
Parution
2015-12-16
Marque
Springer
EAN papier
9784431557685
EAN PDF
9784431557692

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
7
Taille du fichier
4055 Ko
Prix
94,94 €
EAN EPUB
9784431557692

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
7
Taille du fichier
3246 Ko
Prix
94,94 €